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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has become noticed in several tumors, which leads to chromosome instability, and therefore may lead to tumorigenesis. This protein was found to associate with, and be associated with, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in mobile-cycle controlled histone gene expression and plays a crucial job in promoting mobile-cycle development during the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinctive isoforms, happen to be described. Two extra splice variants were being noted but thorough nucleotide sequence details is just not still offered.
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Blocking peptides are peptides that bind particularly on the goal antibody and block antibody binding. These peptide commonly includes the epitope recognized with the antibody.
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This guideline outlines further more doable optimization of cellular and immunoassay parameters to make sure the very best final results are...
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This information outlines even further probable optimization of mobile and immunoassay parameters to make sure the absolute best final results are...
Antibodies bound to the blocking peptide no longer bind on the epitope over the concentrate on protein. By evaluating the staining through the blocked antibody compared to the antibody on your own, one can see which staining is specific.
Target Information and facts Cyclin E belongs into the remarkably conserved cyclin relatives, whose customers are characterized by a dramatic periodicity in protein abundance with the mobile cycle. Cyclins function as regulators of CDK kinases. Distinct cyclins exhibit distinctive expression and degradation designs which contribute to your temporal coordination of every mitotic occasion. Cyclin E sorts a fancy with and functions being a regulatory subunit of CDK2, whose action is necessary for cell cycle G1/S changeover. This protein accumulates within the G1-S phase boundary and is also degraded as here cells development via S section.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by the two antibodies...
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